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Clinical Chemistry, Vol 33, 2209-2213, Copyright © 1987 by American Association for Clinical Chemistry
A Foti, S Kimura, V DeQuattro and D Lee
Department of Medicine, USC School of Medicine, Los Angeles 90033.
In this general method for measuring the concentrations of neurotransmitters in body fluids by "high performance" liquid chromatography (HPLC), the procedures for extracting different biogenic amines from body fluids vary, but the basic chromatographic conditions are the same for all. We use citric/formic acid buffer as the mobile phase, a C18 column, and an electrochemical detector. Specificity is better than in previously reported methods. Because of its excellent cost efficiency, we consider this to be the method of choice for quantifying biogenic amines.
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