Clinical Chemistry
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Clinical Chemistry 33: 2275-2277, 1987;
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Clinical Chemistry, Vol 33, 2275-2277, Copyright © 1987 by American Association for Clinical Chemistry

A monoclonal antibody-based enzyme-linked immunosorbent assay of alpha 1-acid glycoprotein

NH Fraeyman, EJ Van de Velde and FH De Smet
Heymans Institute of Pharmacology, University of Ghent, Medical School, Belgium.

A "sandwich"-type enzyme-linked immunosorbent assay for determining concentrations of human alpha 1-acid glycoprotein (AGP) is described. Microtiter plates coated with a polyclonal rabbit antibody to human AGP were subsequently incubated with the antigen, with a specific murine monoclonal antibody, and with goat anti-mouse immunoglobulins conjugated to alkaline phosphatase. To evaluate the method for assay of AGP in human sera, we compared it with single radial immunodiffusion and "rocket" electroimmunoassay. The respective correlations were r = 0.988 (n = 45) and r = 0.973 (n = 47). Repeated assays of a human serum sample with an average AGP concentration of 859 mg/L yielded within-day and between-day CVs of 1.4% (n = 5) and 6.3% (n = 10), respectively. Because of its low detection limit (4.4 micrograms/L), this assay is also suitable for determination of AGP concentrations in other biological fluids, such as dialysates of patients being treated by continuous ambulatory peritoneal dialysis.




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Copyright © 1987 by the American Association for Clinical Chemistry.