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Clinical Chemistry 33: 2292-2295, 1987;
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Clinical Chemistry, Vol 33, 2292-2295, Copyright © 1987 by American Association for Clinical Chemistry

An alternative fluorescence enhancement solution for use in lanthanide- based time-resolved fluoroimmunoassays

JA Keelan, JT France and PM Barling
Department of Obstetrics and Gynaecology, National Women's Hospital, Auckland, New Zealand.

Time-resolved fluoroimmunoassays that make use of lanthanide chelates as labels require the addition of an enhancement solution to elicit the formation of a fluorescent lanthanide complex. All solutions previously described are based on 2-naphthoyltrifluoroacetone (NTA), a beta- diketone. Currently, this compound is not commercially available. We report here the properties and performance of an enhancement solution prepared with a commercially available beta-diketone, thenoyltrifluoroacetone. Use of this solution in a commercial time- resolved fluoroimmunoassay gave results essentially identical to those obtained with the NTA-based solution, although fluorescence emission was approximately 27% lower. The lower fluorescence yield did not, however, significantly reduce assay sensitivity. We conclude that this solution represents a viable and highly economical alternative to the preparation currently in use, particularly for laboratories wishing to develop their own assays based on lanthanide fluorescence.




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Copyright © 1987 by the American Association for Clinical Chemistry.