Clinical Chemistry
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Clinical Chemistry 33: 253-255, 1987;
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Clinical Chemistry, Vol 33, 253-255, Copyright © 1987 by American Association for Clinical Chemistry

Determination of inorganic fluoride in blood with a fluoride ion- selective electrode

E Kissa

Inorganic fluoride in whole blood, serum, or plasma has been determined with a fluoride ion-selective electrode by adding the 0.5- to 2.0-mL sample to 20 mL of water containing a buffer and fluoride, 25 micrograms/L. The fluoride concentration in the sample is calculated from the resulting cell potential difference recorded after equilibrating for 10 min. This novel method has the advantages of simplicity, accuracy, and high precision, standard deviations for 5 to 7 replicate determinations of fluoride ranging from +/- 1.7 to +/- 2.4 micrograms/L. For whole blood from donors living in an area with fluorinated drinking water, the F- concentration was 20 to 60 micrograms/L.


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J.S. Schultz
Chemical Sensors and Biosensors for Fluoride: Current Status and Developing Technologies
Advances in Dental Research, June 1, 1994; 8(1): 100 - 104.
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Copyright © 1987 by the American Association for Clinical Chemistry.