Clinical Chemistry
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Clinical Chemistry 33: 288-289, 1987;
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Clinical Chemistry, Vol 33, 288-289, Copyright © 1987 by American Association for Clinical Chemistry

Improved separation of fetal hemoglobin by mini-column chromatography

LG Morin and EG Barton

We present chromatographic conditions for separating and measuring fetal hemoglobin on a 5-cm column of Sephadex CM-50. A distinct fetal band is evident, even when present at less than 1% of total hemoglobin. Precision (CV) of better than 5% is achievable.





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Copyright © 1987 by the American Association for Clinical Chemistry.