Clinical Chemistry
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Clinical Chemistry 33: 453-457, 1987;
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Clinical Chemistry, Vol 33, 453-457, Copyright © 1987 by American Association for Clinical Chemistry

Gas chromatographic-mass spectrometric assay for 6-hydroxymelatonin sulfate and 6-hydroxymelatonin glucuronide in urine

PL Francis, AM Leone, IM Young, P Stovell and RE Silman

Circulating melatonin is hydroxylated to 6-hydroxymelatonin and excreted in urine as the sulfate and glucuronide conjugates. We extracted these two compounds from urine by using octadecylsilane- bonded silica cartridges to eliminate most of the urea and electrolytes, and silica cartridges to separate the sulfate and glucuronide conjugates. After hydrolyzing the separated conjugates enzymically, we determined the free hydroxymelatonin by gas chromatography-mass spectrometry. Though recoveries were low and variable, we were able to quantify the analyte in the original sample by adding deuterated sulfate and glucuronide conjugates to the urines before extraction.


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Copyright © 1987 by the American Association for Clinical Chemistry.