Clinical Chemistry
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Clinical Chemistry 33: 498-501, 1987;
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Clinical Chemistry, Vol 33, 498-501, Copyright © 1987 by American Association for Clinical Chemistry

An accelerated enzyme immunoassay for human choriogonadotropin in urine, involving reflow of specimen through capillary tubes

HM Chandler, SA Fuller, CH Nakagawa, PA Nagainis and JG Hurrell

A very rapid and sensitive assay for human choriogonadotropin (hCG) has been developed involving two beta-subunit-specific monoclonal antibodies. In the assay the test specimen is passed backward and forward (reflow) through a monoclonal-antibody-coated capillary tube for 1 min, then incubated for 1 min with a second monoclonal antibody conjugated to urease (EC 3.5.1.5). After addition of a urease substrate solution, 10 int. units of hCG per liter can be detected visually within 5 min, which compares very favorably with other currently available hCG assay procedures. Advantages of the reflow/capillary tube assay system and optimization of the test procedure are discussed.




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Copyright © 1987 by the American Association for Clinical Chemistry.