Clinical Chemistry
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Clinical Chemistry 33: 536-538, 1987;
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Clinical Chemistry, Vol 33, 536-538, Copyright © 1987 by American Association for Clinical Chemistry

Radioimmunoassay of somatomedin-C in filter paper discs containing dried blood

ML Mitchell, RJ Hermos and AC Moses

We describe a simple radioimmunoassay (RIA) for estimating concentrations of somatomedin-C (Sm-C) in dried blood on filter paper. A single 3.2-mm blood spot specimen on filter paper is eluted overnight into buffer containing antibody and 125I-labeled Sm-C. The following day, bound and free hormones are separated by addition of goat anti- rabbit gamma globulin in 60 g/L polyethylene glycol solution. The correlation between values obtained for such blood-spot discs and the corresponding wet plasma is highly significant (r = 0.90, p less than 0.001). The relative concentrations (arbitrary units) of Sm-C as determined for specimens on filter paper from mothers and infants, and for cord bloods, are similar to those reported by others using acidified serum.


The following articles in journals at HighWire Press have cited this article:


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 J. Clin. Endocrinol. Metab.Home page
M. L. Mitchell and Hermos;
Comment on Dried Blood Spot Assay of IGF-I and IGFBP-3
J. Clin. Endocrinol. Metab., February 1, 1999; 84(2): 822 - 822.
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 J. Clin. Endocrinol. Metab.Home page
J. Khosravi and A. Diamandi
Dried Blood Spot Assay of Insulin-Like Growth Factor (IGF)-I and IGF Binding Protein-3 (IGFBP-3)--Authors' Response
J. Clin. Endocrinol. Metab., February 1, 1999; 84(2): 822a - 823.
[Full Text]


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Copyright © 1987 by the American Association for Clinical Chemistry.