Clinical Chemistry
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Clinical Chemistry 33: 561-563, 1987;
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Clinical Chemistry, Vol 33, 561-563, Copyright © 1987 by American Association for Clinical Chemistry

A simple silver-staining technique for detecting Bence Jones proteins in unconcentrated urine

JM Sheat and MA Lorier

Detection of Bence Jones proteins in urine usually involves a concentration step, followed by electrophoresis and, if necessary, immunofixation. The time-consuming and expensive concentration step can be eliminated by use of the silver-stain technique described here. This procedure, routinely used for staining unconcentrated urine, is inexpensive, sensitive, and easily performed in a clinical laboratory. Bence Jones proteins can be detected in concentrations as low as 5 mg/L.




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Copyright © 1987 by the American Association for Clinical Chemistry.