Clinical Chemistry
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Clinical Chemistry 33: 577-578, 1987;
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Clinical Chemistry, Vol 33, 577-578, Copyright © 1987 by American Association for Clinical Chemistry

Comparison of Coomassie Brilliant Blue protein dye-binding assays for determination of urinary protein concentration

T Marshall and KM Williams

We investigated the influence of assay protocol upon results of Coomassie Brilliant Blue protein dye-binding assays for urinary protein. Sensitivity and working range for, and linearity of response (including dilution dependence) of, three standard assays and four micro assays to bovine albumin, gamma-globulin, and urine are compared. The Read and Northcote standard assay (Anal Biochem 1981; 116:53-64) appears to be the method of choice.


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T. Marshall and K. M. Williams
Total Protein Determination in Urine: Elimination of a Differential Response between the Coomassie Blue and Pyrogallol Red Protein Dye-binding Assays
Clin. Chem., March 1, 2000; 46(3): 392 - 398.
[Abstract] [Full Text] [PDF]


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Copyright © 1987 by the American Association for Clinical Chemistry.