Clinical Chemistry
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Clinical Chemistry 33: 604-605, 1987;
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Clinical Chemistry, Vol 33, 604-605, Copyright © 1987 by American Association for Clinical Chemistry

Improved radioimmunoassay of melatonin in serum

W Sieghart, E Ronca, G Drexler and S Karall

Melatonin was extracted from serum by using Baker reversed-phase C-18 columns. More than 99% of the applied melatonin was retained by the columns, and more than 97% was eluted from the columns in 300 microL of methanol. We then determined melatonin in the serum extract by a modification of a standard radioimmunoassay, using filtration instead of centrifugation to collect the [3H]melatonin-antibody complex precipitated by saturated ammonium sulfate. These modifications allow more rapid, accurate, and reproducible determination of melatonin than do previously published procedures.


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 Clin. Chem.Home page
S. Shavali, M. Samejima, K. Uchida, Y. Morita, and A. Fukuda
Improved Enzyme Immunoassay Method for Melatonin: Application to the Determination of Serum Melatonin in Rats, Sheep, and Humans
Clin. Chem., May 1, 1999; 45(5): 690 - 692.
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Copyright © 1987 by the American Association for Clinical Chemistry.