Clinical Chemistry
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Clinical Chemistry 33: 609-611, 1987;
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Clinical Chemistry, Vol 33, 609-611, Copyright © 1987 by American Association for Clinical Chemistry

Enzyme-linked immunosorbent assay for urinary albumin at low concentrations

BK Kramer, U Jesse, KM Ress, RM Schmulling and T Risler

We describe an enzyme-linked immunosorbent assay (ELISA) for urinary albumin. It requires only commercially available reagents, can detect as little as 16 micrograms of albumin per liter, and analytical recovery ranges from 92 to 116%. The assay is simple, rapid, and inexpensive. Albumin excretion was 6.2 (SD 4.1) mg/24 h in healthy subjects (n = 40), 14.7 (SD 7.2) mg/24 h in albumin-test-strip-negative Type I diabetics (n = 11), and 19.7 (SD 16.2) mg/24 h in patients with essential hypertension (n = 12).


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A. J. Bakker, A. Bierma-Ram, H. Keidel, H. Syperda, and A. Zijlstra
(Micro)Albuminuria: Antigen Excess Detection in the Roche Modular Analyzer
Clin. Chem., June 1, 2005; 51(6): 1070 - 1071.
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Copyright © 1987 by the American Association for Clinical Chemistry.