Clinical Chemistry
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Clinical Chemistry 33: 1137-1140, 1987;
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Clinical Chemistry, Vol 33, 1137-1140, Copyright © 1987 by American Association for Clinical Chemistry

One-tube radiotransinassay for determination of cortisol at ambient temperature

BE Murphy and A Barta

After extraction of the sample (on filter paper) in the counting vial, cortisol is assayed by adding 50 microL of horse serum containing tritiated cortisol, and 2 mL of toluene scintillator, shaking for 20 min, and counting the radioactivity in a liquid-scintillation counter at ambient temperature. The method can be applied to saliva, serum, or urine--directly as wet samples or dried on the filter paper. Only inexpensive reagents, which are stable for months to years, are needed.


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Home page
 Transcultural PsychiatryHome page
F. R. Ervin, R. M. Palmour, B. E. Pearson Murphy, R. Prince, and R. C. Simons
The Psychobiology of Trance: II: Physiological and Endocrine Correlates
Transcultural Psychiatry, January 1, 1988; 25(4): 267 - 284.
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Copyright © 1987 by the American Association for Clinical Chemistry.