Clinical Chemistry
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Clinical Chemistry 33: 1177-1184, 1987;
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Clinical Chemistry, Vol 33, 1177-1184, Copyright © 1987 by American Association for Clinical Chemistry

A calibrated fluorescence polarization assay for assessment of fetal lung maturity [published erratum appears in Clin Chem 1988 Jan;34(1):207]

JC Russell

The mechanism of a new fluorescence polarization method for measuring phospholipid in amniotic fluid is described. The fluorescence polarization of a surfactant fluorescent dye solubilized in amniotic fluid correlates significantly with the L/S ratio. Model studies indicate that this results primarily from partitioning of the dye between endogenous albumin, which causes a high fluorescence polarization, and dispersed phospholipid, which causes a much lower polarization. The fluorescence polarization can be compared with a calibration curve to give the ratio of phospholipid to albumin in the sample. This procedure may prove useful in antenatal assessment of fetal lung maturity.


The following articles in journals at HighWire Press have cited this article:


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 Clin. Chem.Home page
D. G. Grenache, C. A. Parvin, and A. M. Gronowski
Preanalytical Factors That Influence the Abbott TDx Fetal Lung Maturity II Assay
Clin. Chem., June 1, 2003; 49(6): 935 - 939.
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R. Cariappa, C. A. Parvin, and A. M. Gronowski
Bilirubin in Amniotic Fluid Does Not Interfere with the Abbott TDx FLM II Assay
Clin. Chem., June 1, 2003; 49(6): 986 - 987.
[Full Text] [PDF]


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Copyright © 1987 by the American Association for Clinical Chemistry.