Clinical Chemistry
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Clinical Chemistry 33: 1235-1236, 1987;
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Clinical Chemistry, Vol 33, 1235-1236, Copyright © 1987 by American Association for Clinical Chemistry

Differential assay of salivary and pancreatic alpha-amylase in serum and urine, with use of monoclonal antibody to human salivary amylase immobilized on bacterial cell wall

S Hiroishi, S Matsuyama, S Kurooka, N Sunahara, S Inoue and T Ida

We previously reported (Clin Chim Acta 1986;159:89) that bacterial cell wall chemically coated with a monoclonal antibody specific to human salivary (S) amylase (EC 3.2.1.1) could be successfully used to separate S and pancreatic (P) amylase in solution. We have now applied this method to serum and urine samples and found that the activities of S and P amylases so measured correlated well with those measured by the isoamylase inhibitor method. The present method is simple and reliable for routine clinical tests.




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Copyright © 1987 by the American Association for Clinical Chemistry.