Clinical Chemistry
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Clinical Chemistry 33: 1242-1245, 1987;
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Clinical Chemistry, Vol 33, 1242-1245, Copyright © 1987 by American Association for Clinical Chemistry

New dip-and-read test for determining leukocytes in urine

AC Skjold, LR Stover, JH Pendergrass, PF Corey, JA Cattell, AE Burkhardt, MP Farinelli, RJ Tyhach, JP Albarella and RW Trimmer

A new reagent strip for the determination of leukocytes in urine (LEUKOSTIX; Ames) is described. The test is based on the esterase activity in leukocytes as a marker. Upon contact between the reagent matrix and a urine containing leukocytes, an amino acid ester is hydrolyzed by the esterase to its corresponding alcohol. The free alcohol then couples with a diazonium salt to produce a purple azo dye. The relative concentration of leukocytes in the urine is obtained by visually comparing the strip reaction with a color chart. Performance of the strip was evaluated in a clinical study involving eight different sites and 867 urine specimens. The comparison method was sediment microscopy; specimens containing five cells or more per high- power field were considered to be positive. Sensitivity was 76.3%, specificity 80.8%. Performance was comparable with that of the CHEMSTRIP LN (Boehringer-Mannheim Diagnostics, Inc.) leukocyte test, which we evaluated concurrently.




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Copyright © 1987 by the American Association for Clinical Chemistry.