Clinical Chemistry, Vol 33, 1397-1402, Copyright © 1987 by American Association for Clinical Chemistry

Precision of glucose measurements in control sera by isotope dilution/mass spectrometry: proposed definitive method compared with a reference method

O Pelletier and C Arratoon

This improved isotope-dilution gas chromatographic/mass spectrometric (GC/MS) method, in which [13C]glucose is the internal standard, meets the requirements of a Definitive Method. In a first study with five reconstituted lyophilized sera, a nested analysis of variance of GC/MS values indicated considerable among-vial variation. The CV for 32 measurements per serum ranged from 0.5 to 0.9%. However, concentration and uncertainty values (mmol/L per gram of serum) assigned to one serum by the NBS Definitive Method (7.56 +/- 0.28) were practically identical to those obtained with the proposed method (7.57 +/- 0.20). In the second study, we used twice more [13C]glucose diluent to assay four serum pools and two lyophilized sera. The CV ranged from 0.26 to 0.5% for the serum pools and from 0.28 to 0.59% for the lyophilized sera. In comparison, results by the hexokinase/glucose-6-phosphate dehydrogenase reference method agreed within acceptable limits with those by the Definitive Method but tended to be slightly higher (up to 3%) for lyophilized serum samples or slightly lower (up to 2.5%) for serum pools.

The following articles in journals at HighWire Press have cited this article:

				A. D M Stork, H. Kemperman, D W. Erkelens, and T. F Veneman Comparison of the accuracy of the hemocue glucose analyzer with the Yellow Springs Instrument glucose oxidase analyzer, particularly in hypoglycemia Eur. J. Endocrinol., August 1, 2005; 153(2): 275 - 281. [Abstract] [Full Text] [PDF]

				U. Hannestad and A. Lundblad Accurate and precise isotope dilution mass spectrometry method for determining glucose in whole blood Clin. Chem., May 1, 1997; 43(5): 794 - 800. [Abstract] [Full Text] [PDF]