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Clinical Chemistry 33: 1619-1623, 1987;
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Clinical Chemistry, Vol 33, 1619-1623, Copyright © 1987 by American Association for Clinical Chemistry

HLA-B27 typing by use of flow cytofluorometry

J Albrecht and HA Muller

We describe the use of flow cytofluorometry to type lymphocytes for HLA- B27 antigens. We modified the cytotoxicity test of Terasaki and McClelland [Nature (London) 1964;204:998] for use with a flow cytofluorometer. In a second assay we used a commercially available monoclonal anti-HLA-B27 antibody and indirect immunofluorescence staining of cell surfaces. Although this antibody cross reacts with B7 and Bw22 antigens, the results for HLA-B27 and the cross-reacting antigens can be separated. Comparison of our results with those by a HLA-typing laboratory for 100 patients showed total agreement of the assigned typings.


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J. J. M. L. Hoffmann and W. C. M. Janssen
HLA-B27 phenotyping with flow cytometry: further improvement by multiple monoclonal antibodies
Clin. Chem., October 1, 1997; 43(10): 1975 - 1981.
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Copyright © 1987 by the American Association for Clinical Chemistry.