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Clinical Chemistry 34: 103-105, 1988;
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Clinical Chemistry, Vol 34, 103-105, Copyright © 1988 by American Association for Clinical Chemistry

A high-speed liquid-chromatographic method for measuring urinary porphyrins

PM Johnson, SL Perkins and SW Kennedy
Division of Biochemistry, Ottawa Civic Hospital, Ontario, Canada.

We describe a rapid quantitative and qualitative "high-performance" liquid-chromatographic (HPLC) method for measuring porphyrins in urine. Direct injection of acidified, filtered urine onto a 3-micron (particle size) 3-cm-long reversed-phase column fully resolves uroporphyrin, hepta-, hexa-, and pentacarboxylic acid porphyrins, and coproporphyrin. Instrument response is linearly related to concentration over the range 25 to 300 nmol/L. The method provides data essential for the differential diagnosis of porphyric states, including porphyria variegata and porphyria cutanea tarda. This relatively inexpensive method requires a run time of only 8 min per sample, making it particularly suitable for routine use in the clinical chemistry laboratory.


The following articles in journals at HighWire Press have cited this article:


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 Annals of Clinical & Laboratory ScienceHome page
K. L. Nuttall
Abnormal Chromatographic Patterns of Porphyrins in Urine
Ann. Clin. Lab. Sci., July 1, 2001; 31(3): 259 - 264.
[Abstract] [Full Text] [PDF]

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 Clin. Chem.Home page
J. T. Hindmarsh, L. Oliveras, and D. C. Greenway
Plasma Porphyrins in the Porphyrias
Clin. Chem., July 1, 1999; 45(7): 1070 - 1076.
[Abstract] [Full Text] [PDF]


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