Clinical Chemistry
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Clinical Chemistry 34: 106-109, 1988;
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Clinical Chemistry, Vol 34, 106-109, Copyright © 1988 by American Association for Clinical Chemistry

Simple, rapid enzymatic determination of glycerophosphocholine in human seminal plasma

HJ Chap, JP Moatti, R Mieusset, M Nieto, G Laneelle, PJ Bennet, A Mansat, F Pontonnier and L Douste-Blazy
Laboratoire de Biochimie III, Hopital La Grave, Toulouse, France.

We have devised a new enzymatic determination of sn-glycero-3- phosphocholine (GPC) in human seminal plasma. This is based on GPC hydrolysis by a phosphodiesterase (PDE), free choline being then determined by the choline oxidase method. The whole procedure involves a first incubation in the presence of choline oxidase and catalase, to eliminate the excess of choline present in seminal plasma (10-fold, compared with GPC). Absorbance and concentration are linearly related up to at least 100 nmol per assay, analytical recovery ranges between 89% and 105%, and intra- and interassay CVs are 3.2% and 5.6%, respectively, at the highest substrate concentration. Using this procedure, we found seminal plasma from 21 fertile men to contain 5.22 (SD 3.33) mumol per ejaculate--within the same range as previously reported values obtained chromatographically. After vasectomy, GPC in seminal plasma decreased to 28% of its original value, as determined in 10 volunteers. Thus this new method displays appropriate characteristics of specificity, reliability, and convenience, allowing its use in routine evaluation of male fertility.


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C. Delagebeaudeuf, A. Gassama-Diagne, M. Nauze, A. Ragab, R. Y. Li, J. Capdevielle, P. Ferrara, J. Fauvel, and H. Chap
Ectopic Epididymal Expression of Guinea Pig Intestinal Phospholipase B. POSSIBLE ROLE IN SPERM MATURATION AND ACTIVATION BY LIMITED PROTEOLYTIC DIGESTION
J. Biol. Chem., May 29, 1998; 273(22): 13407 - 13414.
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Copyright © 1988 by the American Association for Clinical Chemistry.