Clinical Chemistry
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Clinical Chemistry 34: 152-154, 1988;
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Clinical Chemistry, Vol 34, 152-154, Copyright © 1988 by American Association for Clinical Chemistry

Effects of non-ionic and anionic detergents on lipid-associated tissue ferritin

BE Cham, P Roeser and A Nikles
Department of Medicine, University of Queensland, Royal Brisbane Hospital, Australia.

Lipid-associated ferritin from homogenates of guinea pig liver is released from its conjugate(s) by incubation with the non-ionic detergents Triton X-100 and Nonidet P-40 but not by incubation with the anionic detergent deoxycholate. The amount of lipid-associated ferritin released from its conjugate(s) depends on the concentration of the non- ionic detergents. At a final non-ionic detergent concentration of about 20 g/L, all lipid-associated ferritin is released from its conjugate(s) in a liver homogenate. The amount released is identical with the amount of the lipid-associated ferritin obtained by extraction of the same liver homogenate with a mixture of butanol and diisopropyl ether.




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Copyright © 1988 by the American Association for Clinical Chemistry.