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Clinical Chemistry 34: 53-58, 1988;
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Clinical Chemistry, Vol 34, 53-58, Copyright © 1988 by American Association for Clinical Chemistry

Effect of lot-to-lot variability in filter paper on the quantification of thyroxin, thyrotropin, and phenylalanine in dried-blood specimens

WE Slazyk, DL Phillips, BL Therrell Jr and WH Hannon
Division of Environmental Health Laboratory Sciences, Centers for Disease Control, Atlanta, GA 30333.

We prepared whole-blood pools to contain various concentrations of phenylalanine (Phe), thyroxin (T4), and thyrotropin (TSH) and applied them to six different lots of Schleicher & Schuell Grade 903 filter paper, two of which represented extremes for serum-absorbancy. Individual measured T4 values showed minimal overlap among all pools for each individual filter-paper lot and for all lots combined, but Phe values overlapped considerably among the high-concentration pools within and among lots. Individual TSH values also showed considerable overlap among the high-concentration pools for all lots combined, but little overlap within each lot. Maximum differences in mean observed values among lots ranged from 6% to 36% for all analytes. Assay results from hemolyzed blood specimens generally were lower than from intact- cell blood specimens for T4 and TSH, but slightly higher for Phe. Maximum among-lot differences in mean values ranged from 13% to 29% for all analytes when each tested lot was used for assay calibration. Lot- to-lot differences in measured values were not strongly related to serum absorbancy values. We conclude that routinely encountered within- and among-lot filter paper variability, as measured by serum- absorbancy, is not alone sufficient to cause gross quantification errors in neonatal screening programs.


The following articles in journals at HighWire Press have cited this article:


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 Am. J. Clin. Nutr.Home page
S. D O'Broin and E. W Gunter
Screening of folate status with use of dried blood spots on filter paper
Am. J. Clinical Nutrition, September 1, 1999; 70(3): 359 - 367.
[Abstract] [Full Text] [PDF]


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Copyright © 1988 by the American Association for Clinical Chemistry.