Clinical Chemistry
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Clinical Chemistry 34: 63-66, 1988;
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Clinical Chemistry, Vol 34, 63-66, Copyright © 1988 by American Association for Clinical Chemistry

Time-resolved immunofluorometric assay of sex-hormone binding globulin

S Niemi, O Maentausta, NJ Bolton and GL Hammond
Farmos Diagnostica, Oulunsalo, Finland.

A time-resolved immunofluorometric assay (trlFMA) for human sex-hormone binding globulin (SHBG) is described in which antibody-coated tubes or microliter strip-wells and a europium (Eu) chelate-labeled monoclonal antibody are used. The trlFMA sensitivity is similar to that of other SHBG immunoassays, and other analytical variables compare favorably with an SHBG immunoradiometric assay (IRMA) kit and a steroid binding capacity assay: the interassay coefficient of variation (CV) is less than 8% and the intra-assay CV is less than 6% for concentrations between 6 and 200 nmol/L. The reference intervals (means +/- SD) for SHBG concentrations (nmol/L) in serum from 10 men, 10 women, and 10 pregnant women were 23 +/- 12, 65 +/- 39, and 439 +/- 122, respectively. In 14 hirsute women the mean +/- SD serum SHBG concentration (37 +/- 21 nmol/L) was significantly lower (P less than 0.01) than the mean for an age-matched, nonhirsute female comparison group. The trlFMA is technically simple, requires no centrifugation or separation reagent, and takes a counting time of only 1 s. In addition, the Eu-label is nontoxic, presents no waste-disposal problems, and has a long shelf life.


The following articles in journals at HighWire Press have cited this article:


Home page
 J Mol EndocrinolHome page
D. M Selva and G. L Hammond
Thyroid hormones act indirectly to increase sex hormone-binding globulin production by liver via hepatocyte nuclear factor-4{alpha}
J. Mol. Endocrinol., July 1, 2009; 43(1): 19 - 27.
[Abstract] [Full Text] [PDF]

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 EndocrinologyHome page
D. M. Selva and G. L. Hammond
Peroxisome-Proliferator Receptor {gamma} Represses Hepatic Sex Hormone-Binding Globulin Expression
Endocrinology, May 1, 2009; 150(5): 2183 - 2189.
[Abstract] [Full Text] [PDF]

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 J. Clin. Endocrinol. Metab.Home page
D. M. Selva, L. Bassas, F. Munell, A. Mata, F. Tekpetey, J. G. Lewis, and G. L. Hammond
Human Sperm Sex Hormone-Binding Globulin Isoform: Characterization and Measurement by Time-Resolved Fluorescence Immunoassay
J. Clin. Endocrinol. Metab., November 1, 2005; 90(11): 6275 - 6282.
[Abstract] [Full Text] [PDF]

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 J. Biol. Chem.Home page
D. M. Selva, K. N. Hogeveen, and G. L. Hammond
Repression of the Human Sex Hormone-binding Globulin Gene in Sertoli Cells by Upstream Stimulatory Transcription Factors
J. Biol. Chem., February 11, 2005; 280(6): 4462 - 4468.
[Abstract] [Full Text] [PDF]


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Copyright © 1988 by the American Association for Clinical Chemistry.