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Clinical Chemistry 34: 67-70, 1988;
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Clinical Chemistry, Vol 34, 67-70, Copyright © 1988 by American Association for Clinical Chemistry

Direct spectrophotometry of bilirubin in serum of the newborn, with use of caffeine reagent

KL Vink, W Schuurman and R van Gansewinkel
Department of Clinical Chemistry, St. Joseph Ziekenhuis, Eindhoven, The Netherlands.

We recently described a direct spectrophotometric method for unconjugated bilirubin, with caffeine reagent (Clin Chem 1986;32:1389- 93). Because this method is independent of the protein matrix we used it for the preparation of bilirubin standards (Clin Chem 1987;33:1817- 21). Now, in this paper, we utilize the caffeine reagent in setting up a bilirubin method for serum from neonates. This resulted in a two- wavelength (465 and 528 nm) equation, which fully corrects for HbO2 interferences. In combination with a bilirubin standard, this equation may be transformed into a simple relative formula for use with this simple dilution method. We studied this two-wavelength method with 55 neonates' sera, comparing results with those by both the diazo method of Doumas et al. (Clin Chem 1985;31:1779-89) and the borate method of Hertz et al. (Scand J Clin Lab Invest 1974;33:215-30). We found that this new method is independent of hemolysis and of the matrix of the sera. Therefore, it is very suitable for use in neonatology.


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S. C. Kazmierczak, A. F. Robertson, P. G. Catrou, K. P. Briley, B. L. Kreamer, and G. R. Gourley
Direct Spectrophotometric Method for Measurement of Bilirubin in Newborns: Comparison with HPLC and an Automated Diazo Method
Clin. Chem., July 1, 2002; 48(7): 1096 - 1097.
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