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Clinical Chemistry 34: 2005-2008, 1988;
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Clinical Chemistry, Vol 34, 2005-2008, Copyright © 1988 by American Association for Clinical Chemistry

Development of a direct assay for alpha-amylase

ES Winn-Deen, H David, G Sigler and R Chavez
Behring Diagnostics, Somerville, NJ 08876.

We describe a direct colorimetric assay for alpha-amylase, with 2- chloro-4-nitrophenyl-alpha-maltotrioside as substrate. Both human pancreatic and salivary amylase split this substrate without the use of helper enzymes, yielding free 2-chloro-4-nitrophenol, which is monitored at 405 nm. The performance of this reagent compares well with that of Pantrak Amylase (Behring Diagnostics) for both serum and urine samples. The reagent is very stable in dry powder form and is stable for one month at 2 to 8 degrees C after reconstitution. Because of the rapid color development and linear kinetics (less than 30 s), the assay is easily automated. Results can be obtained in less than 5 min.


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[Abstract] [Full Text]


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Copyright © 1988 by the American Association for Clinical Chemistry.