Clinical Chemistry
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Clinical Chemistry 34: 2053-2057, 1988;
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Clinical Chemistry, Vol 34, 2053-2057, Copyright © 1988 by American Association for Clinical Chemistry

Evaluation of an enzyme immunoassay kit for estrogen receptor measurement--report II

S Raam and DM Vrabel
Tufts University School of Medicine, Tufts University Medical Cancer Unit at Lemuel Shattuck Hospital, Boston, MA 02130.

We present evidence to show that monoclonal antibodies to estrogen receptors (ER) in solid phase recognize the secondary estrogen binding sites with moderate to low affinity for estradiol (E2). An excellent quantitative agreement was found in five cytosols between the ER values obtained by the enzyme immunoassay (ER-EIA) and the amount of secondary estrogen binding sites measured by the assay involving dextran-coated charcoal (Clin Chem 1986;32:1496). The immunoreactive protein recognized by the antibody-coated beads, when allowed to react with ER(+) cytosols, is shown to bind [3H]estradiol only when the ligand concentration exceeds 8 nmol/L. Further biochemical and functional characterization of the immunoreactive protein is required to establish similarities/dissimilarities between this protein, high-affinity Type I ER sites, and the secondary sites such as Type II sites.




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Copyright © 1988 by the American Association for Clinical Chemistry.