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Clinical Chemistry, Vol 34, 2125-2126, Copyright © 1988 by American Association for Clinical Chemistry
JW van den Berg, R Koole-Lesuis, A Edixhoven-Bosdijk and N Brouwers
Erasmus University, Department of Internal Medicine II, Rotterdam, The Netherlands.
We present a modification of the HemoQuant assay, a good but lengthy and tedious method for determining heme in feces by means of its transformation to porphyrins. The laborious extraction procedure was replaced by a simple centrifugation procedure. The nonhomogeneous hot oxalic acid suspension was replaced by acetic acid. We observed no significant difference in results between samples analyzed by the older method vs the present modification (r = 0.996, n = 52). Mean (and SD) analytical recoveries of added hemoglobin and protoporphyrin were 99% (7%) and 93% (6%), respectively. The analytical procedure can now be automated by using discrete samplers and a flow-through fluorometer. Initial sampling and dilution of feces are still done manually, however. The excellent specificity, sensitivity, and overall analytical performance of the original method are retained, while circumventing the practical inconveniences of this reliable screening test for occult blood in feces.
The following articles in journals at HighWire Press have cited this article:
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F. Pierre, A. Freeman, S. Tache, R. Van der Meer, and D. E. Corpet Beef Meat and Blood Sausage Promote the Formation of Azoxymethane-Induced Mucin-Depleted Foci and Aberrant Crypt Foci in Rat Colons J. Nutr., October 1, 2004; 134(10): 2711 - 2716. [Abstract] [Full Text] [PDF]
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F. Pierre, S. Tache, C. R. Petit, R. Van der Meer, and D. E. Corpet Meat and cancer: haemoglobin and haemin in a low-calcium diet promote colorectal carcinogenesis at the aberrant crypt stage in rats Carcinogenesis, October 1, 2003; 24(10): 1683 - 1690. [Abstract] [Full Text] [PDF]
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A. L. A. Sesink, D. S. M. L. Termont, J. H. Kleibeuker, and R. Van der Meer Red meat and colon cancer: dietary haem-induced colonic cytotoxicity and epithelial hyperproliferation are inhibited by calcium Carcinogenesis, October 1, 2001; 22(10): 1653 - 1659. [Abstract] [Full Text] [PDF]
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A. L.A. Sesink, D. S.M.L. Termont, J. H. Kleibeuker, and R. Van der Meer Red meat and colon cancer: dietary haem, but not fat, has cytotoxic and hyperproliferative effects on rat colonic epithelium Carcinogenesis, October 1, 2000; 21(10): 1909 - 1915. [Abstract] [Full Text] [PDF]
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