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Clinical Chemistry 34: 2130-2133, 1988;
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Clinical Chemistry, Vol 34, 2130-2133, Copyright © 1988 by American Association for Clinical Chemistry

High-molecular-mass alkaline phosphatase: simplified and highly sensitive determination by liquid chromatography

E Schoenau, W Boeswald, KH Herzog, J Scheele, HJ Boehles and K Stehr
Klinik mit Poliklinik fur Kinder und Jugendliche der Friedrich- Alexander Universitat, Erlangen, F.R.G.

This simplified HPLC method for measurement of high-molecular-mass alkaline phosphatase (high-Mr AP; EC 3.1.3.1) in serum and bile is rapid (time for column preparation and separation 30 min), reproducible (CV 4.2%), and highly sensitive (detects high-Mr AP in healthy controls at 1-3% of total AP activity in serum), and is suitable for processing small batches of sample. We characterized high-Mr AP in serum and bile by incubating samples with L-phenylalanine, neuraminidase, 1-butanol, or wheat-germ lectin, and by determining stability to heat. High-Mr AP activity was determined in sera of patients with various liver diseases (4-32% of total AP serum activity) and results were compared with those by electrophoresis on agarose.




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Copyright © 1988 by the American Association for Clinical Chemistry.