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Clinical Chemistry 34: 2205-2207, 1988;
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Clinical Chemistry, Vol 34, 2205-2207, Copyright © 1988 by American Association for Clinical Chemistry

Enzyme immunosorbent assay of prolactin with penicillinase as label

TG Shrivastav, PK Pandey and GL Kumari
Department of Reproductive Biomedicine, National Institute of Health and Family Welfare, New Delhi, India.

This competitive, rapid, and sensitive enzyme immunosorbent assay for measuring human prolactin in plasma involves penicillinase (EC 3.5.2.6) as label. Microtiter plate wells coated with goat anti-rabbit gamma globulin are filled with antibody to prolactin and plasma sample or reference prolactin and incubated with penicillinase-labeled prolactin for 1 h at 37 degrees C. The enzyme activity of the bound complex is then measured. The assay is as sensitive as radioimmunoassay, the detection limit being 2.5 ng of prolactin per milliliter of plasma. The plasma prolactin values obtained by enzyme immunoassay correlated well with those determined by radioimmunoassay: r = 0.98, slope = 1.00, intercept = 1.11 ng/mL (n = 53).


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
A. Basu, T. G. Shrivastav, and K. P. Kariya
Preparation of Enzyme Conjugate through Adipic Acid Dihydrazide as Linker and Its Use in Immunoassays
Clin. Chem., August 1, 2003; 49(8): 1410 - 1412.
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Copyright © 1988 by the American Association for Clinical Chemistry.