Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 34: 2241-2244, 1988;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Atherton, N. D.
Right arrow Articles by Green, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Atherton, N. D.
Right arrow Articles by Green, A.

Clinical Chemistry, Vol 34, 2241-2244, Copyright © 1988 by American Association for Clinical Chemistry

HPLC measurement of phenylalanine in plasma

ND Atherton and A Green
Clinical Chemistry Department, Children's Hospital, Birmingham, U.K.

Measurement of phenylalanine in plasma is required for the diagnosis and subsequent dietary management of phenylketonuria (PKU). We have developed an isocratic high-performance liquid-chromatographic (HPLC) method, with ultraviolet detection. For this measurement 10 microL of plasma is needed, and the complete analysis can be done in less than 15 min. Interbatch coefficients of variation for human plasma samples (phenylalanine 136-1541 mumol/L) ranged between 2.2% and 4.4%. The method has a wide linear range (10-3500 mumol/L), and results (y) correlated well with those (x) of an ion-exchange chromatographic method (r = 0.996; y = 1.014 x - 11.9; n = 64, range 22-1823 mumol/L). Only standard HPLC equipment is needed and no specialized sample preparation or detection system is required. The procedure has proved robust and reliable in regular routine use.


The following articles in journals at HighWire Press have cited this article:


Home page
 J. Biol. Chem.Home page
R. Eavri and H. Lorberboum-Galski
A Novel Approach for Enzyme Replacement Therapy: THE USE OF PHENYLALANINE HYDROXYLASE-BASED FUSION PROTEINS FOR THE TREATMENT OF PHENYLKETONURIA
J. Biol. Chem., August 10, 2007; 282(32): 23402 - 23409.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
M. Huengsberg, J. B. Winer, M. Gompels, R. Round, J. Ross, and M. Shahmanesh
Serum kynurenine-to-tryptophan ratio increases with progressive disease in HIV-infected patients
Clin. Chem., April 1, 1998; 44(4): 858 - 862.
[Abstract] [Full Text] [PDF]

Home page
 JPEN J Parenter Enteral NutrHome page
L. E. Harrison, S. N. Hochwald, M. J. Heslin, R. Berman, M. Burt, and M. F. Brennan
Early Postoperative Enteral Nutrition Improves Peripheral Protein Kinetics in Upper Gastrointestinal Cancer Patients Undergoing Complete Resection: A Randomized Trial
JPEN J Parenter Enteral Nutr, July 1, 1997; 21(4): 202 - 207.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1988 by the American Association for Clinical Chemistry.