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Clinical Chemistry 34: 2299-2301, 1988;
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Clinical Chemistry, Vol 34, 2299-2301, Copyright © 1988 by American Association for Clinical Chemistry

Assay of dipeptidyl peptidase IV in serum by fluorometry of 4-methoxy-2- naphthylamine

S Scharpe, I De Meester, G Vanhoof, D Hendriks, M van Sande, K Van Camp and A Yaron
Faculty of Medicine, University of Antwerp, Wilrijk, Belgium.

A new fluorometric assay for determining dipeptidyl peptidase IV (DPP IV; EC 3.4.14.5) was developed. The synthetic substrate glycyl-L- proline-4-methoxy-2-naphthylamide (20 mmol/L), Tris buffer (50 mmol/L, pH 8.3), and serum (20 microL) are mixed and incubated. The reaction is stopped with citrate (100 mmol/L, pH 4.0) and the released 4-methoxy-2- naphthylamine is measured fluorometrically. The mean value of DPP IV activity in serum for 64 healthy subjects was 58 (SD 16) mumol of 4- methoxy-2-naphthylamine released per liter of serum per minute. The proposed procedure is sensitive, rapid, and accurate and can easily be automated.


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Copyright © 1988 by the American Association for Clinical Chemistry.