Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 34: 2433-2438, 1988;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Knight, J. A.
Right arrow Articles by McClellan, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Knight, J. A.
Right arrow Articles by McClellan, L.

Clinical Chemistry, Vol 34, 2433-2438, Copyright © 1988 by American Association for Clinical Chemistry

Specificity of the thiobarbituric acid reaction: its use in studies of lipid peroxidation

JA Knight, RK Pieper and L McClellan
Department of Pathology, University of Utah School of Medicine, Salt Lake City.

The thiobarbituric acid (TBA) reaction, quantified by colorimetry or fluorimetry, is the method most widely used for studying lipid peroxidation in both laboratory animals and in humans with disorders. However, concerns regarding its analytical specificity have often been expressed, because TBA reacts with a wide variety of chemical species to produce a pink to red color. In this study, we reacted TBA with various saturated and unsaturated aldehydes (both directly and in the presence of sucrose, fructose, and glucose), substituted pyrimidines, 2- deoxyribose, and N-acetylneuraminic acid. We also studied the TBA reaction with bilirubin, biliverdin, icteric serum, and serum containing hemolyzed erythrocytes, comparing the absorption spectra of these reaction products with that for malondialdehyde (MDA). The reaction products were also analyzed for MDA by high-performance liquid chromatography (HPLC). Although the TBA reaction with some of these compounds may not be important in biological studies, others could lead to misinterpretations of increased lipid peroxidation. Use of HPLC to quantify MDA is recommended because of its high analytical sensitivity and specificity, especially in the study of lipid peroxidation in human subjects.


The following articles in journals at HighWire Press have cited this article:


Home page
 J Exp BotHome page
H. H. Ratnayaka, W. T. Molin, and T. M. Sterling
Physiological and antioxidant responses of cotton and spurred anoda under interference and mild drought
J. Exp. Bot., October 1, 2003; 54(391): 2293 - 2305.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
Y.-T. Lee, L.-Y. Chiang, W.-J. Chen, and H.-C. Hsu
Water-Soluble Hexasulfobutyl[60]fullerene Inhibit Low-Density Lipoprotein Oxidation in Aqueous and Lipophilic Phases
Experimental Biology and Medicine, June 1, 2000; 224(2): 69 - 75.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
M. J. Cox, H. S. Sood, M. J. Hunt, D. Chandler, J. R. Henegar, G. M. Aru, and S. C. Tyagi
Apoptosis in the left ventricle of chronic volume overload causes endocardial endothelial dysfunction in rats
Am J Physiol Heart Circ Physiol, April 1, 2002; 282(4): H1197 - H1205.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1988 by the American Association for Clinical Chemistry.