Clinical Chemistry
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Clinical Chemistry 34: 2528-2532, 1988;
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Clinical Chemistry, Vol 34, 2528-2532, Copyright © 1988 by American Association for Clinical Chemistry

Concomitant purification of prostatic carcinoma tumor markers from human seminal fluid under nondenaturing conditions

D Rusciano, A Berardi, C Ceccarini and B Terrana
Cellular Biology Laboratory, Sclavo Research Center, Siena, Italy.

We report a protocol for concomitant purification to homogeneity of both prostatic acid phosphatase [orthophosphoric-monoester phosphohydrolase (acid optimum), EC 3.1.3.2] and prostate-specific antigen, from human seminal fluid. The method requires only two chromatographic steps: passage through an Affigel-Blue column and gel filtration HPLC. This is a fast, efficient procedure for purification of these two important tumor markers, which are specific for prostatic cancer.




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Copyright © 1988 by the American Association for Clinical Chemistry.