Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 34: 261-264, 1988;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Primus, F. J.
Right arrow Articles by Goldenberg, D. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Primus, F. J.
Right arrow Articles by Goldenberg, D. M.

Clinical Chemistry, Vol 34, 261-264, Copyright © 1988 by American Association for Clinical Chemistry

"Sandwich"-type immunoassay of carcinoembryonic antigen in patients receiving murine monoclonal antibodies for diagnosis and therapy

FJ Primus, EA Kelley, HJ Hansen and DM Goldenberg
Center for Molecular Medicine and Immunology, University of Medicine and Dentistry of New Jersey, Newark 07103.

Measurements of carcinoembryonic antigen (CEA) in blood increased dramatically in some patients who were receiving injections of monoclonal antibody. CEA titers were measured with a monoclonal antibody-based double-determinant enzyme immunoassay in which untreated plasma specimens were diluted with an equal volume of buffer containing mouse serum. Increasing CEA titers were accompanied by the appearance and coincident increase in titers of human antibody against mouse Ig (HAMA). Adsorption of these sera with solid-phase anti-human IgG or Protein A restored antigen titers to pretreatment values; evidently the serum factor eliciting false-positive CEA titers was most probably HAMA. Neither addition of undiluted mouse serum to the assay mixture nor pretreatment by heating plasma specimens to 70 degrees C effectively abolished HAMA interference. By contrast, protein precipitation with polyethylene glycol (130 g/L) or heating plasma samples to 90 degrees C eliminated false-positive titers caused by HAMA, but did not reduce authentic CEA titers.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
J. Bjerner, O. P. Bormer, and K. Nustad
The War on Heterophilic Antibody Interference
Clin. Chem., January 1, 2005; 51(1): 9 - 11.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
J. Bjerner, K. Nustad, L. F. Norum, K. H. Olsen, and O. P. Bormer
Immunometric Assay Interference: Incidence and Prevention
Clin. Chem., April 1, 2002; 48(4): 613 - 621.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
L. J. Kricka
Human Anti-Animal Antibody Interferences in Immunological Assays
Clin. Chem., July 1, 1999; 45(7): 942 - 956.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
J.-F. Benoist, D. Orbach, and D. Biou
False increase in C-reactive protein attributable to heterophilic antibodies in two renal transplant patients treated with rabbit antilymphocyte globulin
Clin. Chem., September 1, 1998; 44(9): 1980 - 1985.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
J. Reinsberg
Interferences with Two-site Immunoassays by Human Anti-mouse Antibodies Formed by Patients Treated with Monoclonal Antibodies: Comparison of Different Blocking Reagents
Clin. Chem., August 1, 1998; 44(8): 1742 - 1744.
[Full Text] [PDF]

Home page
 Clin. Chem.Home page
N. Despres and A. M. Grant
Antibody interference in thyroid assays: a potential for clinical misinformation
Clin. Chem., March 1, 1998; 44(3): 440 - 454.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1988 by the American Association for Clinical Chemistry.