Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 34: 370-371, 1988;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Surmont, D. W.
Right arrow Articles by Alexandre, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Surmont, D. W.
Right arrow Articles by Alexandre, J. A.

Clinical Chemistry, Vol 34, 370-371, Copyright © 1988 by American Association for Clinical Chemistry

Adaptations to keep a thyrotropin immunoradiometric assay "supersensitive" with automated pipetting

DW Surmont and JA Alexandre
Department of Nuclear Medicine, Universite Libre de Bruxelles (U.L.B.), Hopital Erasme, Belgium.

The sensitivity of the immunoradiometric kit "RIA-gnost hTSH" (Behring) is poorer with a common automated pipetting station than with careful manual pipetting, principally because of tracer contamination of the inner wall of the tube, above the level reached by the wash solution. This can be eliminated by adapting the wash buffer volume so as to fill the entire tube. In this way, one can detect as little thyrotropin as 0.02 milli-int. unit/L, which is better than the 0.03 milli-int. unit/L claimed by the manufacturer. Cross-contamination from pipetting can induce overestimation of "suppressed" thyrotropin values. Prolonging the counting time to obtain an optimal counting error is of no practical value in the thyrotropin range where activity is low.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1988 by the American Association for Clinical Chemistry.