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Clinical Chemistry 34: 416-418, 1988;
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Clinical Chemistry, Vol 34, 416-418, Copyright © 1988 by American Association for Clinical Chemistry

Immunoassay of low concentrations of albumin in urine by latex particle counting

CL Cambiaso, D Collet-Cassart and M Lievens
Unit of Experimental Medicine, International Institute of Cellular and Molecular Pathology, Brussels, Belgium.

We describe here a nonisotopic immunoassay, based on particle-counting technology, for the determination of urinary albumin. The assay takes only 35 min and has been fully automated on the IMPACT (Acade Diagnostic Systems, Brussels, Belgium) machine. The system measures albumin within a linear range between 6.25 and 50 mg/L and has a detection limit of 0.4 mg/L. Analytical recoveries at three concentrations ranged between 96% and 102%. Within-run precision ranged from 1.6% to 9.5%. The method was compared with a commercial nephelometric immunoassay system and a correlation coefficient of 0.996 was found for 216 urine samples. No antigen excess affects the shape of the curve in our system, whereas in nephelometry a 3 g/L solution of albumin starts to decrease the dose-response curve.




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Copyright © 1988 by the American Association for Clinical Chemistry.