Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 34: 902-905, 1988;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Moscoso, H.
Right arrow Articles by Garver, F. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Moscoso, H.
Right arrow Articles by Garver, F. A.

Clinical Chemistry, Vol 34, 902-905, Copyright © 1988 by American Association for Clinical Chemistry

Quantification of hemoglobins S, C, and F by a magnetic affinity immunoassay

H Moscoso, CR Kiefer, A Kutlar and FA Garver
Department of Cell and Molecular Biology, Medical College of Georgia, Augusta 30912-2100.

This magnetic affinity immunoassay (MAIA) quantifies hemoglobins (Hb) S, C, and F in hemolysates from adults or newborns. Monospecific antisera to the hemoglobins are covalently conjugated to magnetic beads and reacted with the corresponding 125I-labeled hemoglobin. After centrifugation to separate the free and antibody-bound 125I-labeled hemoglobin, the amount of radioactive hemoglobin in the pellet is measured. To determine the concentration of the Hb under study, the percent inhibition of the reaction is quantified. The standard curve is established by adding known quantities of unlabeled hemoglobin before adding 125I-labeled hemoglobin. The amount of Hb S, Hb C, or Hb F present in hemolysates is determined by measuring the percentage of inhibition and extrapolating the concentration from the standard curve. Results agree well with values for Hb S and Hb C obtained by "high- performance" liquid chromatography and RIA and for Hb F as measured by alkali denaturation and RIA. This assay can be completed in 1 h and is more sensitive than enzyme immunoassay or RIA: we can detect proportions of Hb S and Hb C as low as 1% of total Hb, and Hb F as low as 0.05%.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1988 by the American Association for Clinical Chemistry.