Clinical Chemistry
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Clinical Chemistry 34: 1011-1017, 1988;
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Clinical Chemistry, Vol 34, 1011-1017, Copyright © 1988 by American Association for Clinical Chemistry

Pseudouridine is unsuitable as an endogenous renal clearance marker

JT Bernert Jr, CJ Bell, J Guntupalli and WH Hannon
Division of Environmental Health Laboratory Sciences, Centers for Disease Control, Atlanta, GA 30333.

We assessed the clearance of endogenous pseudouridine in humans to evaluate the potential use of this modified nucleoside as a marker of glomerular filtration rate. Pseudouridine concentrations in serum ultrafiltrates and in the corresponding 24-h urine specimens from 19 healthy men were determined by high-performance liquid chromatography. Mean (and SD) pseudouridine concentrations in serum and urine from this group averaged 2.77 (0.34) mumol/L and 203.2 (64.8) mumol/L, respectively. The calculated clearances of the nucleoside [87.3 (24.9) mL/min, n = 19], however, averaged approximately one-third lower than the corresponding creatinine clearances in the same individuals [131.8 (28.4) mL/min]. Measurement of simultaneous clearances of [3H]pseudouridine and [14C]inulin in rats also yielded a lower pseudouridine clearance, 0.78 relative to inulin. Our results are thus consistent with a partial net reabsorption of pseudouridine in both experimental animals and in humans, indicating that this compound would not be a suitable endogenous marker for routine estimation of the glomerular filtration rate.




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Copyright © 1988 by the American Association for Clinical Chemistry.