Clinical Chemistry
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Clinical Chemistry 34: 1018-1021, 1988;
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Clinical Chemistry, Vol 34, 1018-1021, Copyright © 1988 by American Association for Clinical Chemistry

Solid-phase radioimmunoassay of human complement fragment C5a [published errata appear in Clin Chem 1988 Nov;34(11):2370 and 1989 Apr;35(4):670]

S Chang, A Leo-Mensah, J Campbell, M Stastny and RA Patrick
Ciba-Corning Diagnostics, E. Walpole, MA 02032.

In this competitive RIA for determining concentrations of human C5a in biological fluids and in buffers, labeled C5a and sample are allowed to compete for binding to a limited amount of goat antibody to human C5a in solution. Free and bound tracer are then separated by a second antibody (rabbit anti-goat IgG) immobilized on paramagnetic particles. Total incubation time for this assay is 70 min. Sensitivity, precision, and analytical recovery of this assay compare well with those of a reference method.




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Copyright © 1988 by the American Association for Clinical Chemistry.