Clinical Chemistry
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Clinical Chemistry 34: 1062-1066, 1988;
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Clinical Chemistry, Vol 34, 1062-1066, Copyright © 1988 by American Association for Clinical Chemistry

Development of standard materials for use with the hematofluorometer

PJ Parsons, JR Meola and DG Mitchell
Laboratory of Inorganic and Nuclear Chemistry, Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201.

Two methods for preparing erythrocytes were evaluated for the purpose of developing stable, blood-based standard materials for the hematofluorometer. Erythrocytes washed with citrate-phosphate-dextrose solution and reconstituted with platelet-free plasma were stable for 12 weeks. Plasma-free erythrocytes washed with isotonic saline and resuspended in citrate-glycerol solution were stable for 18 weeks when stored at 4 degrees C. Consequently, plasma-free erythrocytes were successfully used as proficiency test specimens in the New York State Department of Health's Clinical Laboratory Evaluation Program for hematofluorometer users.




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Copyright © 1988 by the American Association for Clinical Chemistry.