Clinical Chemistry
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Clinical Chemistry 34: 1119-1121, 1988;
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Clinical Chemistry, Vol 34, 1119-1121, Copyright © 1988 by American Association for Clinical Chemistry

Multi-wavelength analyses of second-derivative spectra for rapid determination of acetaminophen in serum

B Dingeon, MA Charvin, MT Quenard and H Thome
Service de Biologie, Centre Hospitalier Chambery, France.

Measurement of acetaminophen by analysis of the second derivative of its spectrum is specific and sensitive. The method of extraction and the use of just one phosphate buffer as reagent makes this method very convenient. Readings are reliable from 10 to 1500 mg/L. A turnaround time of 20 min makes this method well suited for emergency cases. Precision and accuracy of the method are presented. Results are not biased by interferences, not even from N-acetylcysteine.




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Copyright © 1988 by the American Association for Clinical Chemistry.