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Clinical Chemistry 34: 1124-1127, 1988;
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Clinical Chemistry, Vol 34, 1124-1127, Copyright © 1988 by American Association for Clinical Chemistry

Revised calibration of the Reflotron cholesterol assay evaluated

GJ Boerma, I van Gorp, TL Liem, B Leijnse, J Bellm and CA Carstensen
Department of Clinical Chemistry, Academic Hospital Rotterdam, The Netherlands.

We evaluated the Boehringer Mannheim (B.M.) Reflotron Total Cholesterol "dry-chemistry" method after its recalibration in 1987. Reports in the literature up to 1986-1987 of a negative bias (up to -10%) in the method prompted a revision of the factory-set calibration of the Reflotron. For this, B.M. prepared a new set of calibrators with 12 different concentrations of cholesterol. We checked in two ways whether accuracy had been achieved: (a) The values assigned to the calibrators by B.M. were checked with the manual Abell-Kendall Reference Method (MAK) performed in an official Reference Center. These were shown to be correct. (b) Concurrently, a direct comparison was made by analyzing 200 fresh samples of human serum. Reflotron cholesterol values obtained for these samples proved to be accurate, meeting the current World Health Organization/Centers for Disease Control criterion of maximum bias less than or equal to 5%. Orthogonal regression analysis yielded the following correlation: Reflotron = 0.985 MAK + 0.238 mmol/L (y = ax + b). Reflotron mean = 6.26 mmol/L; MAK mean = 6.09 mmol/L. SDa = 0.015 mmol/L; SDb = 0.120 mmol/L, and r = 0.989.


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G. J. M. Boerma
The Office Measurement of Cholesterol
JAMA, January 20, 1989; 261(3): 382 - 382.
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