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Clinical Chemistry 34: 1459-1461, 1988;
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Clinical Chemistry, Vol 34, 1459-1461, Copyright © 1988 by American Association for Clinical Chemistry

Modified TDx assay for cyclosporine and metabolites, for use with whole- blood samples

W Vogt and I Welsch
Institut fur Klinische Chemie und Laboratoriumsmedizin, Deutsches Herzzentrum Munchen des Freistaates Bayern, F.R.G.

A recently introduced fluorescence polarization immunoassay (FPIA) for determination of cyclosporine A in serum and plasma is discussed with regard to its use for whole-blood samples, with and without hemolysis before the assay. The performance characteristics of the modified method are highly satisfactory (within-run CVs 2.06 to 5.50% and 1.99 to 3.39%, respectively; long-term between-run CVs under routine conditions 5.73 to 8.95%). The limit of detection is 30 micrograms/L. Results agree well with those obtained with the RIAs compared, but the modified FPIA is more convenient and faster.




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Copyright © 1988 by the American Association for Clinical Chemistry.