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Clinical Chemistry 34: 1545-1551, 1988;
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Clinical Chemistry, Vol 34, 1545-1551, Copyright © 1988 by American Association for Clinical Chemistry

Radioimmunoassay of salivary cyclosporine with use of 125I-labeled cyclosporine

JE Coates, SF Lam and WT McGaw
Department of Laboratory Medicine, University of Alberta Hospitals, Edmonton, Canada.

We prepared 125I-labeled cyclosporine (125I-CS) by modifying the procedure of Mahoney and Orf (Clin Chem 1985;31:459) and characterized it with regards to maximal immunoreactivity (greater than 90%), trichloroacetic acid precipitability (greater than 90%), and stability (90% immunoreactive after five half-lives of 125I). For a particular preparation of 125I-CS, we estimated its immunoreaction concentration (50 pmol/L) and the equilibrium constant for its reaction with Sandoz polyclonal antiserum (K = 3.9 X 10(9) L/mol). By substituting 125I-CS as tracer in the Sandoz radioimmunoassay and by modifying other aspects of the assay, we developed a procedure that is sufficiently sensitive (0.34 micrograms/L) to allow measurement of "trough" (lowest inter- dose) cyclosporine concentrations in parotid saliva. Of 38 kidney- transplant patients, 35 had measurable concentrations in saliva (mean 8.3, SD 5.2 micrograms/L), and these correlated moderately with paired serum concentrations (r = 0.68, P less than 0.001). We believe that measurement of salivary cyclosporine may offer a simple way of estimating the free fraction of the drug in serum or plasma.


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E. Kaufman and I. B. Lamster
THE DIAGNOSTIC APPLICATIONS OF SALIVA-- A REVIEW
Critical Reviews in Oral Biology & Medicine, March 1, 2002; 13(2): 197 - 212.
[Abstract] [Full Text] [PDF]


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Copyright © 1988 by the American Association for Clinical Chemistry.