Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 34: 1561-1564, 1988;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Phillipou, G.
Right arrow Articles by Phillips, P. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Phillipou, G.
Right arrow Articles by Phillips, P. J.

Clinical Chemistry, Vol 34, 1561-1564, Copyright © 1988 by American Association for Clinical Chemistry

Re-evaluation of the fructosamine reaction

G Phillipou, CJ Seaborn and PJ Phillips
Endocrine and Diabetes Laboratory, Queen Elizabeth Hospital, Woodville, South Australia.

The difference in spectral characteristics between 1-deoxy-1- morpholinofructose (DMF) and protein/plasma samples in the fructosamine reaction has been related to the solubility of the diformazan formed by reduction of nitro blue tetrazolium chloride. Addition of the surfactant Triton X-100 (20 g/L) to the reagent buffer not only corrects this anomaly but also enhances the absolute response. Detailed investigation of DMF and dihydroxyacetone as calibration standards for the reaction established a clear preference for the latter. Fundamental differences in reaction kinetics were also noted between the Amadori rearrangement products of glucose formed from morpholine (DMF) or the amino lysine groups of protein (glycated albumin). From the reactivity of dihydroxyacetone, as well as glyceraldehyde, observed in the fructosamine reaction, and the presence of this class of compounds (trioses) in human plasma, we infer that they may also contribute to the differentiation of diabetic and non-diabetic samples.


The following articles in journals at HighWire Press have cited this article:


Home page
 Diabetes CareHome page
D. E. Goldstein, R. R. Little, R. A. Lorenz, J. I. Malone, D. Nathan, C. M. Peterson, and D. B. Sacks
Tests of Glycemia in Diabetes
Diabetes Care, July 1, 2004; 27(7): 1761 - 1773.
[Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1988 by the American Association for Clinical Chemistry.