Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 34: 1689-1692, 1988;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Phillips, T. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Phillips, T. M.

Clinical Chemistry, Vol 34, 1689-1692, Copyright © 1988 by American Association for Clinical Chemistry

High-performance immunoaffinity chromatographic detection of immunoregulatory anti-idiotypic antibodies in cancer patients receiving immunotherapy

TM Phillips
Department of Medicine, George Washington University Medical Center, Washington, DC 20037.

Anti-idiotypic antibodies are regulatory antibodies responsible for the shutdown of active immune responses against growing tumor cells. In an attempt to study these antibodies, a technique for isolating specific anti-idiotypic antibodies by immunoaffinity chromatography was devised. Human anti-tumor antibodies were isolated by affinity absorption to fixed autologous tumor cells. These antibodies were biotinylated, immobilized on streptavidin-coated beads, and used as a ligand to isolate reactive anti-idiotypes from the plasma of patients during periods when immune reactivity against their tumors could not be detected. The isolated anti-idiotypes demonstrated the ability to react with the original antibodies and to inhibit their binding to autologous tumor cells. Thus functional anti-idiotypic antibodies can be isolated by immunoaffinity chromatography with the original idiotype as the ligand. This technique can be used to monitor regulatory antibodies in cancer patients receiving immunotherapy.


The following articles in journals at HighWire Press have cited this article:


Home page
 Clin. Chem.Home page
D. S. Hage
Affinity Chromatography: A Review of Clinical Applications
Clin. Chem., May 1, 1999; 45(5): 593 - 615.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1988 by the American Association for Clinical Chemistry.