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Clinical Chemistry 34: 1720-1725, 1988;
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Clinical Chemistry, Vol 34, 1720-1725, Copyright © 1988 by American Association for Clinical Chemistry

A non-flow cytometric system for detecting antibodies and cellular antigens in blood

V Ghazarossian, M Laney, J Vorpahl, J Pease, C Skold, R Watts, H Jeong, A Dafforn, R Cook and EF Ullman
Syva Co., Research Department, Palo Alto, CA 94303.

The system described here can distinguish between single and agglutinated erythrocytes by use of a non-flow fiber optic fluorometer. The method is capable of detecting cell-surface antigens and antibodies to cell-surface antigens present in blood. Significant features include high-efficiency fluorescent dyes that intercalate into cell membranes, a stretching membrane for transport and mixing of samples, charged colloidal magnetite for magnetic separation of erythrocytes, and an immersible fiber optic probe for measuring fluorescence associated with cells in a 1-nL volume of a bulk solution. We describe the application of the system to automation of ABO/Rh grouping and antibody screening.




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Copyright © 1988 by the American Association for Clinical Chemistry.