Clinical Chemistry Link to Randox Laboratories Web Site
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 34: 1758-1762, 1988;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Alfthan, H.
Right arrow Articles by Stenman, U. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Alfthan, H.
Right arrow Articles by Stenman, U. H.

Clinical Chemistry, Vol 34, 1758-1762, Copyright © 1988 by American Association for Clinical Chemistry

Choriogonadotropin and its beta subunit separated by hydrophobic- interaction chromatography and quantified in serum during pregnancy by time-resolved immunofluorometric assays

H Alfthan, J Schroder, R Fraser, A Koskimies, H Halila and UH Stenman
Department of Obstetrics and Gynecology, Helsinki University Central Hospital, Finland.

Concentrations of human choriogonadotropin (hCG) and its free beta subunit (beta hCG) were measured in serum by highly sensitive and specific time-resolved immunofluorometric assays (IFMAS). The results were confirmed by completely separating beta hCG and hCG by a novel method based on hydrophobic-interaction chromatography. We used three monoclonal antibodies in two different combinations. In both assays an antibody reacting with both free beta hCG and with intact hCG was immobilized onto the wall of a microtiter strip well. For assay of intact hCG we used as the indicator antibody an antibody against the alpha subunit, labeled with a europium chelate. For assay of beta hCG we used an indicator antibody that reacted only with the free beta subunit. hCG cross-reacted in the assay of beta hCG by 0.6%. Quantifying hCG in serum after in vitro fertilization showed that, seven to eight days after embryo transfer, the hCG concentration started to increase, thereafter increasing with a doubling time of 1.9 days during the following three weeks. hCG concentrations in serum peaked six to 10 weeks later, corresponding to eight to 12 weeks after the last menstrual period. Throughout pregnancy, measurable amounts of beta hCG were present in serum. The highest beta hCG/hCG ratio (maximum 7.3%, median 3.0%) was observed during early gestation. During the fourth to 13th weeks after the last menstrual period the ratio of beta hCG/hCG decreased gradually, being 1.0% during the second and third trimesters.


The following articles in journals at HighWire Press have cited this article:


Home page
 Hum Reprod UpdateHome page
U.-H. Stenman, A. Tiitinen, H. Alfthan, and L. Valmu
The classification, functions and clinical use of different isoforms of HCG
Hum. Reprod. Update, November 1, 2006; 12(6): 769 - 784.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
L. Kangasniemi, T. Kiviluoto, A. Kanerva, M. Raki, T. Ranki, M. Sarkioja, H. Wu, F. Marini, K. Hockerstedt, H. Isoniemi, et al.
Infectivity-Enhanced Adenoviruses Deliver Efficacy in Clinical Samples and Orthotopic Models of Disseminated Gastric Cancer.
Clin. Cancer Res., May 15, 2006; 12(10): 3137 - 3144.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
P. von Lode, J. Rainaho, and K. Pettersson
Quantitative, Wide-Range, 5-Minute Point-of-Care Immunoassay for Total Human Chorionic Gonadotropin in Whole Blood
Clin. Chem., June 1, 2004; 50(6): 1026 - 1035.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
J. Korhonen, H. Alfthan, P. Ylostalo, J. Veldhuis, and U.-H. Stenman
Disappearance of human chorionic gonadotropin and its {alpha}- and ß-subunits after term pregnancy
Clin. Chem., November 1, 1997; 43(11): 2155 - 2163.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
U.-H. Stenman, L. Unkila-Kallio, J. Korhonen, and H. Alfthan
Immunoprocedures for detecting human chorionic gonadotropin: clinical aspects and doping control
Clin. Chem., July 1, 1997; 43(7): 1293 - 1298.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1988 by the American Association for Clinical Chemistry.