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Clinical Chemistry 34: 1767-1771, 1988;
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Clinical Chemistry, Vol 34, 1767-1771, Copyright © 1988 by American Association for Clinical Chemistry

Enzyme-linked immunosorbent assay for serum amyloid A apolipoprotein with use of specific antibodies against synthetic peptides

R Saile, C Delpierre, P Puchois, G Hocke, C Cachera, JC Gesquiere, A Steinmetz, A Tartar and JC Fruchart
Serlia, Institut Pasteur de Lille, France.

We describe a noncompetitive enzyme-linked immunosorbent assay for amyloid A apolipoprotein in human serum (apo SAA) in which specific antibodies against synthetic peptides are used. Microtiter plates were used as solid phase and coated with affinity-purified antibodies raised against SAA1-(95-104) peptide. After incubation with delipidated plasmas, the bound apo SAA was revealed by labeled antibodies raised against SAA1-(58-69) peptide. The assay offers several advantages over existing techniques: sensitivity, specificity, simplicity, and non-use of radioisotopes. Results correlate well with those by a nephelometric method in which polyclonal antibodies are used.




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Copyright © 1988 by the American Association for Clinical Chemistry.