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Clinical Chemistry 34: 1816-1820, 1988;
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Clinical Chemistry, Vol 34, 1816-1820, Copyright © 1988 by American Association for Clinical Chemistry

Totally automated liquid-chromatographic assay for cortisol and associated glucocorticoids in serum, with use of ASTED sample preparation

DC Turnell, JD Cooper, B Green, G Hughes and DJ Wright
Biochemistry Department, Coventry and Warwickshire Hospital, U.K.

We describe estimation of total cortisol in serum by HPLC. Incorporation of a new technique to totally automate sample preparation decreases costs. The benefits of HPLC ensure that interferences from other steroids are avoided. The method can also be used to estimate concentrations of other glucocorticoids in serum.




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Copyright © 1988 by the American Association for Clinical Chemistry.